PREpare is broken down into 2 very distinct concepts.
- Cell Swelling Stack.
- Mental Focus Stack.
For the Cell Swelling Stack, we wanted to include clinical dosages of ingredients that would ensure increased nutrient delivery, improve waste product removal, cell swelling and fullness of the trained muscle and ensure optimal levels of vascularity.
Prepare brings together the addition of patented Setria® Glutathione with 6g of pure L-Citrulline. Nitric oxide (NO) is endogenously synthesized from L-arginine and L-citrulline and due to its effects on the enzyme isoforms of nitric oxide synthase (NOS). The addition of reduced glutathione (GSH) may protect against the oxidative reduction of NO thereby ensuring the production of NO is maintained. Furthermore, an increase in NO production may enhance oxygen and nutrient delivery to active muscle resulting in more efficient anabolism of muscle tissue.
L-arginine is oxidized to synthesize NO. Therefore, it can be hypothesized that increased serum levels of l-arginine can increase NO production under healthy normal conditions. However, arginine has been shown to exhibit poor oral bioavailability. An effective method for addressing issues with arginine bioavailability was found by adding substances to enhance arginine absorption, one such element being silicon. There is some evidence to suggest that silicon, especially as silicic acid, may protect vascular integrity during age-related vascular diseases further adding benefit to its inclusion.
Nitrosogine™, a patented inositol-stabilized arginine silicate has been shown to increase blood levels of arginine and silicon in both an acute and chronic dosing regimen. After a single 1,500 mg dose of an arginine silicate supplement, levels of arginine increased significantly for up to 5 hours and levels of silicon for up to 1.5 hours. Prepare includes this clinical 1,500mg dose of arginine silicate.
Typically, powdered forms of glycerol are included in pump forumals in the form of glycerol monostearate (GMS) which contains at most 25% glycerol and the remainder being composed of fats. HydroMax® is a patented, highly concentrated form of powdered glycerol and silica, composed of 65% glycerol leading to an end result of exceptional hydration and superior pumps compared to traditional GMS.
Muscles expel taurine during contraction and it is known that a 32% greater increase in plasma taurine levels occurs in a hydrated state which has a role in osmotic regulation.
The Cell Swelling Stack of PREpare contains 5g Hydromax® and 1,500mg Taurine to support and maintain cellular hydration; helping to increase visible vascularity by acting as “hyper-hydrating agents” to increase blood flow to muscles.
Unlike other stimulant based products, the main goal of PREpare’s Mental Focus Stack is to support neurotransmitter production and drive cognitive performance.
L-Tyrosine is the precursor of the catecholamines; alterations in the availability of L-tyrosine to the brain can influence the synthesis of both dopamine and norepinephrine. Consuming the amino-acid tyrosine, the precursor of dopamine and norepinephrine, may counteract decrements in neurotransmitter function and cognitive performance. Physical performance can benefit from Tyrosine supplementation under specific conditions that activate catecholamine neurons and recruit higher cognitive functions which places high enough cognitive demands on the individual that induce catecholamine depletion. Therefore, the main effects of L-tyrosine supplementation that have been reported are acute effects in preventing a decline in cognitive function in response to physical stress.
Dietary choline levels are linked to the rate of biosynthesis of acetylcholine. Given that cholinergic nerves trigger muscle contraction, and that choline availability is linked to acetylcholine synthesis, ingesting substances that could augment choline availability might have the potential to influence muscular performance.
Ingested Alpha-Glycerylphosphorylcholine (A-GPC) is converted to phosphatidylcholine (a source of choline) which could potentially augment human performance by facilitating neuro-muscular interaction and boosting the release of acetylcholine to stimulate cognitive functioning.
Huperzine A is a potent, selective and reversible acetylcholinesterase inhibitor which inhibits the enzyme acetylcholinesterase which breaks down acetylcholine. This ensures that acetylcholine will not be broken down as readily and will remain longer in the body prolonging potential cognitive performance benefits yielded from A-GPC.
As such the Mental Focus Stack of PREpare contains 300mg Patented Alphasize® Alpha-Glyceryl Phosphoryl Choline (Alpha-GPC) yielding 150mg A-GPC and 200mcg Huperzine A.
The majority of research has examined the effects of moderate to high caffeine doses (5–13 mg/kg body mass) on exercise and sport. These caffeine doses have profound effects on the responses to exercise at the whole-body level and are associated with variable results and some undesirable side effects.
Low doses of caffeine (<3 mg/kg body mass, ~250 mg for 85kg BM) are also ergogenic in some exercise and sport situations, although this has been less well studied.
Studies using high doses of caffeine (5–13 mg/kg bm) report ergogenic effects in endurance-type activities, but there are also pronounced effects on the physiological responses to exercise, including increased heart rates, a doubling of the catecholamine levels, higher blood lactate levels, and increased blood free fatty acid (FFA) and glycerol levels in many subjects. Also, the ingestion of high caffeine doses can also produce troubling side effects of gastrointestinal upset, nervousness, mental confusion, inability to focus, and disturbed sleeping in some subjects, especially those who are habitual light caffeine users.
The administration of a low caffeine dose (3 mg/kg bm) can also produce an ergogenic effect, with no changes in exercise heart rate and the levels of catecholamines, lactate, FFA, and glycerol which strongly suggests that the ergogenic effect of caffeine is mediated through the central nervous system (CNS).
Low doses of caffeine (~200 mg) have also been shown to improve vigilance, alertness and mood, and improve cognitive processes during and following strenuous exercise. For this reason we decided to include just enough caffeine in PREPARE at 250mg to keep energy maximized with minimal crash.
TrainedbyJP Nutrition – PREpare to challenge yourself.
Formulated alongside Dr Dean St Mart
Per 18g Serving:
Cell Swelling Stack
- 6000mg L-Citrulline
- 5000mg Hydromax® (Patented glycerol complex yielding 65% gycerol)
- 1500mg Nitrosogine® (Patented inositol-stabilized arginine silicate)
- 1500mg Taurine
- 200mg Setria® (Patented Glutathione)
Mental Focus Stack
- 1500mg L-Tyrosine
- 300mg Alphasize® (Patented Alpha-GPC yielding 150mg Alpha-Glyceryl Phosphoryl Choline)
- 250mg Caffeine
- 200mcg Huperzine A
McKinley-Barnard, Andre T, Morita M, Willoughby DS. Combined L-citrulline and glutathione supplementation increases the concentration of markers indicative of nitric oxide synthesis. J Int Soc Sports Nutr. 2015;12:27.
Cuisinier C, Michotte De Welle J, Verbeeck RK, Poortmans JR, Ward R, Sturbois X, Francaux M. Role of taurine in osmoregulation during endurance exercise. Eur J Appl Physiol. 2002;87:489–495.
Schwedhelm E. et al. Pharmacokinetic and pharmacodynamic properties of oral L-citrulline and L-arginine: impact on nitric oxide metabolism. Br J Clin Pharmacol. 2008 Jan; 65(1): 51–59.
Bellar D, LeBlanc N R., Campbell B. The effect of 6 days of alpha glycerylphosphorylcholine on isometric strength. J Int Soc Sports Nutr. 2015; 12: 42.
Young S. L-Tyrosine to alleviate the effects of stress? J Psychiatry Neurosci. 2007 May; 32(3): 224.
Jongkees B. et al. Effect of tyrosine supplementation on clinical and healthy populations under stress or cognitive demands—A review. J. Psychiatr. Res. November 2015. Volume 70:50-57.
Zhang H, New insights into huperzine A for the treatment of Alzheimer’s disease. Acta Pharmacol Sin. 2012 Sep; 33(9): 1170–1175.
Spriet, L. Exercise and Sport Performance with Low Doses of Caffeine. Sports Med. 2014; 44(Suppl 2): 175–184.